728x90 생물학실험2 [생물학실험] Midi prep protocol [ Midi prep ] standard protocol Step 1. Spread plate (comp cell + DNA + LB/ 1 hour) Step 2. Mini culture (pick the colony & put into LB(3ml)+AMP(3ul)/ shaking) Step 3. Midi culture (put 100-200ul mini culture in LB 100ml (500ml flask)/ shaking overnight) Step 4. Midi prep (using kit) Preparation 1. Autoclave 완료한 500ml flask 2. LB media 1) LB-media(in 시약냉장고) 4capsule/1L (autoclave 된 500ml 플라스크 사용.. 2022. 12. 28. [생물학실험] Maxi prep protocol (one DNA) [ Maxi prep ] standard protocol Step 1. Spread plate (comp cell + DNA + LB/ 1 hour) Step 2. Mini culture (pick the colony & put into LB(3ml)+AMP(3ul)/ shaking) Step 3. Midi culture (put 100-200ul mini culture in LB 100ml (500ml flask)/ shaking overnight) Step 4. Midi prep (using kit) Preparation 1. Autoclave 완료한 500ml flask 2. LB media 1) LB-media(in 시약냉장고) 4capsule/1L (autoclave 된 500ml 플라스크 사용.. 2022. 12. 28. 이전 1 다음 728x90